To Mary Anne Theresa Whitby1 2 September 
Down Farnborough Kent
Your great kindness in giving me last year at Southampton2 information on the varieties on the silk-worm, makes me venture once again to trouble you. My question is a very simple one, and yet I am very curious to have it answered on the best authority.— Whenever I have observed the moths raised from silk-worm kept by children, the wings have been more or less crumpled, & I have been assured that they can never fly. Does this hold good, especially in France & Italy? If it does, can you, inform me, whether the males & females are equally helpless as regards flight?3 I presume that they are in the same condition, as our domestic ducks, & I should be extremely grateful for any information on this point.—
You were so kind last year as to give me hopes that you would try two experiments on hereditariness (a point on which I am particularly interested) in the caterpillar state: the first was whether the black eye-browed kind would produce black or dark-eyed caterpillar children:4 the second was to see if the very fat caterpillars (which I think you called Frales & which you described to me in a very laughable manner) would produce moths; & if so whether their offspring would be likewise fat & silkless.—5 I can really hardly say, how grateful I should be to know the results of such experiments; for in a work which I intend some few years hence to publish on variation, there will be hardly any facts in the insect world.
Will you permit me one other question, namely whether you have ever observed any difference in habits, such as in manner of crawling, eating, spinning &c in the caterpillars of the different breeds, which you have kept.— I am well aware I have much reason to apologise for thus presuming to trouble you, & I can only trust to your kindness to excuse.
Pray believe, dear Madam, with much respect. | Your sincerely obliged | Charles Darwin
Questions Mrs W on difference in flight capacity of male and female silkworm moths and asks her for results of experiments he suggested she do with silkworms to determine hereditariness of dark "eyebrows". [See Variation 1: 302.]